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Tsan-Piao Lin, Professor
Position Professor
Highest Degree

Ph.D., 1985 Crop Science Department, Oregon State University


Plant stress biology, Seed biology, Plant molecular evolution

Laboratory Life Science Building R1047
Telephone 02-33662537
Research Area :
  1. Plant stress biology: Searching for regulatory genes that confer drought tolerance to Arabidopsis thaliana

  2. Seed biology

  3. Native orchid resource
Professional experience :

1985/9 – 1987/8 Post doctoral associate
          Department of Biochemistry, Michigan State University
1987/9 – 2000/7 Taiwan Forestry Research Institute, Taipei
1997/7 – 1998/1 Visiting scientist, Cornell University

Research Interests :
Searching for regulatory genes that confer drought tolerance to Arabidopsis thaliana
Abiotic stresses, such as drought, salinity, extreme temperatures, chemical toxicity and oxidative stress are serious threats to agriculture and is the primary cause of crop loss worldwide, reducing average yields for most major crop plants by more than 50%. However desiccation/drought stress tolerant plants exist in different places, and genes confer stress tolerance are everywhere. Desiccation of seeds is considered to be a prerequisite for the completion of life cycle; desiccation of pollen is considered necessary for the dispersal by wind, and limited plants (resurrection plant) acquire desiccation tolerance in the vegetative growth. Desiccation/drought tolerance is the result of a dynamic process and appears to be mediated by the protective systems that prevent lethal damage. It also the result of the cooperation of physiological and biochemical alterations at the cellular and molecular levels, for example, the accumulation of various osmolytes and soluble sugars, late embryogenesis abundant proteins, efficient antioxidant systems in seeds and pollens. We have been using Saleginella tamariscina, a native lower vascular plant, as material to investigate the mechanism of desiccation tolerance.

The knowledge of regulatory genes and an understanding of their mode of action will provide an important starting point for the improvement of crop plants to face the abotic environment. The discovery and use of new stress-tolerance-associated genes to confer plant stress tolerance has been the subject of ongoing efforts internationally to obtain tolerant plants.
Studies demonstrated the important role of transcription factors in the acquisition of stress tolerance. Currently, we direct our attention to characterize the function of the AtDS1, AtERF53, AtERF1 and AtAIL7 all belonging to the AP2/ERF family. They either play as a positive or negative regulator of drought and salt stress response.? For example, we found AtDS1 overexpressors are sensitive to dehydration. ABI1 might act as an upstream gene of AtDS1, and can activate the expression of AtDS1 in the presence AtHB6 (see the figure below). The gene expression level of ABI2 was influenced by the expression level of AtDS1. ABI2 might be a direct target of AtDS1. Since no AP2/ERF protein binding cis-element was found in the ABI2 promoter, there must be a new cis-element in the promoter of ABI2 for the binding of AtDS1. These results suggest that AtDS1 is a linker between ABI1 and ABI2, and play a role under drought stress and ABA treatment.
We are also interested in glutathione S-transferase U17 which play a negative regulatory function in the drought/salt stress tolerance in Arabidopsis. The surprisingly discovery of GSTU17 plays a new function in drought/salt stress drives us to screen all the GSTU knockout mutants. We hope to find interesting gene and further characterize their function in abiotic stress.

Selected Publications

    1. Kuo DC, Lin CC, Ho KC, Cheng YP, Hwang SY*, Lin TP* 2010 Two genetic divergence centers revealed by chloroplastic DNA variation in populations of Cinnamomum kanehirae Hay. Conservation Genetics 11:803–812.

    2. Shih MD, Hsieh TY, Lin TP, Hsing YI C*, Hoekstra FA* 2010 Characterization of two soybean (Glycine max L.) LEA IV proteins by circular dichroism and Fourier transform infrared spectrometry. Plant & Cell Physiology 51(3):395-407.

    3. Liao PC, Kuo DC, Lin CC, Ho KH, Lin TP,* Hwang SY* 2010 Historical spatial range expansion and a very recent bottleneck of Cinnamomum kanehirae Hay. (Lauraceae) in Taiwan inferred from nuclear genes. BMC Evolutionary Biology 10:124.

    4. Yang SY, Shih MD, Lin TP, Hsing YIC* 2011 Two soybean (Glycine max L.) GmPM proteins reduce liposome leakage during desiccation. Botanical Stidies 52: 465-470.

    5. Wu HY, Liu MS, Lin TP, Cheng YS* 2011 Structural and functional assays of AtTLP18.3 identify its novel acid phosphatase activity in thylakoid lumen. Plant Physiology 157: 1015–1025.

    6. Chen JH, Jiang HW, Hsieh EJ, Chen HU, Chien CT, Hsieh HL, Lin TP* 2012 Drought and salt stress tolerance of Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid. Plant Physiology 158: 340–351.

    7. Cheng MC, Hsieh EJ, Chen JH, Chen HU, Lin TP* 2012 Arabidopsis RGLG2, functioning as a RING E3 ligase, interacts with AtERF53 and negatively regulates the plant drought stress response. Plant Physiology 158: 363–375.

    8. En-Jung Hsieh EJ, Cheng MC, Lin TP* 2013 Functional characterization of an abiotic stress-inducible transcription factor AtERF53 in Arabidopsis thaliana. Plant Molecular Biology 82:223–237.

    9. Cheng MC, Po-Ming Liao, Wei-Wen Kuo, Lin TP* 2013 The Arabidopsis ETHYLENE RESPONSE FACTOR1 regulates abiotic stress-responsive gene expression by binding to different cis-Acting elements in response to different stress signals. Plant Physiology 162: 1566–1582.

    10. Cheng MC, Ko K, Chang WL, Kuo WC, Chen GH, Lin TP* 2015 Increased glutathione contributes to stress tolerance and global translational changes in Arabidopsis. Plant Journal 83: 926-39.

    11. Chen HY, Hsieh EJ, Cheng MC, Chen CY, Hwang SY, Lin TP* 2016 ORA47 (octadecanoid-responsive AP2/ERF-domain transcription factor 47) regulates jasmonic acid and abscisic acid biosynthesis and signaling through binding to a novel cis-element.New Phytol.

    12. Cheng MC, Kuo WC, Wang YM, Chen HY, Lin TP* 2017 UBC18 mediates ERF1 degradation under light-dark cycles.New Phytol.

Courses taught 
  Plant stress physiology(合開)
Lab Members

Kuan-Wu, Hsin-Chu County Aug 4, 2009

Lab members

Rui-Hung Chen (PhD student)
Characterization of the glutathione s-transferase U17 gene of Arabidopsis thaliana

En-Jung Hsieh (PhD student)
Characterization of a dehydration inducible transcription factor of Arabidopsis

Nina Mei-Chun Cheng (PhD student)
Characterization of RGLG2, an E3 ligase which interacts with the AtERF53

Dr. Hsin-Yu Chen (Postdoc)

Rui-Hung Chen  
En-Jung Hsieh  
Nina Mei-Chun Cheng  
Dr. Hsin-Yu Chen  


Copyright © 2004, Institute of Plant Biology, National Taiwan University

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